Description

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TransfectionReagentforNCI-H292Cells(LungCarcinomaCells,CRL-1848)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroNCI-H292transfectionprotocol:[PDF]
• Download NCI-H292CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforNCI-H292 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast93%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsNCI-H292TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

NCI-H292CellLine:

NCI-H292cellsaresensitiveformanydifferentviruses,includingherpessimplexvirus,measlesvirus,mumpsaswellassomestrainsofinfluenzavirustypeA.ThismakesthemanexcellentsubstituteforMKcellswithregardtolaboratorysafety,paramyxovirusisolation,andeaseofuse,accordingtotheJournalofClinicalMicroBIOLOGy(Jun1993,Vol.31,pp.1504–1510)TheNCI-H292tumOrigeniccelllineisderivedfromalymphnodemetastasislungcellsofa32-year-oldfemalewithmucoepidermoidpulmonarycarcinomaandexhibitsepithelialmorphology.NCI-H292cellshavebeennegativelytestedforL-DOPAdecarboxylaseanddisplaythesamemucoepidermoidcharacteristicsasnormal(non-cancerous)cellsdo.NCI-H292cellssusceptibletotheHepatitisBvirus,makingthemvaluabletoolsforbiomedicalresearch.Thiscelllineisusedasinvitromodelinavarietyofmolecularandcellbiologyresearchrelatedtolungcancer.AltogenBiosystemsmanufactureshigh-efficiencytransfectionreagentkitsforthiscellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheNCI-H292cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHinNCI-H292cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoNCI-H292cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6858)
• 1.5ml(Catalog#6859)
• 1.5mlCRISPR(Catalog#2179)
• 8.0ml(Catalog#7013)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。