Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).
Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).
Transfection Reagent for 3LL Cells (Lewis Lung Carcinoma Cells)
Transfection Efficiency:
Reagent exhibits at least 73% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems 3LL Transfection Protocol: [PDF]
Download MSDS: [PDF]
3LL Cell Line:
Lung cancer is considered the most preventable malignancy globally. However, it is the deadliest form of cancer with very high mortality rates because of lack of adequate therapies and aggressive tumor cell growth. Rodent cell line models play an essential role in preclinical research to study novel therapeutic agents and treating human cancer. The LLC cell line is a well-studied mouse cancer model that is extensively employed as a transplantable malignancy model. Lewis Lung Carcinoma cells (also known as 3LL cells or LLC cells) got their name after Dr. Margaret R. Lewis of the Wistar Institute, who discovered carcinoma in 1951. This cell line has been known to show reduced metastatic ability when exposed to ultraviolet light. It has also been observed that the presence of cannabinoids suppresses cell growth. These cells could be useful for medical research for treatments to certain types of lung cancer, and many other molecular or cell biology studies. According to the recent data, the proliferative advantage of 3LL metastatic cells is achieved by the gene expression which prevents tumor cells from escaping hostile microenvironmental conditions. Altogen Biosystems provides nanoparticle-based transfection kits for this cell line.
Data:
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, 3LL cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
Figure 2. Protein expression of GAPDH in 3LL cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into 3LL cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
Altogen Biosystems:
Altogen Biosystems manufacturers cell type specific and preoptimized transfection products, elecroporation kits, and in vivo delivery reagents for life science research. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
Volume Options:
Altogen Biosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂、癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNA(mRNA,siRNA,shRNA,microRNA),蛋白质和小分子研究。
苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理,Altogen Biosystems华东代理,Altogen Biosystems华北代理,Altogen Biosystems全国代理,Altogen Biosystems华南代理。