Description

ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).

Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).

TransfectionReagentforBHK-21Cells(KidneyCells,CCL-10)

• Abiodegradablepolymerbasedtransfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• AchieverobustsiRNAuptakefordependablegenesilencing
• Effectivetransfectionunderconditionsofupto40%serum
• TransfectionkitincludesTransfectionEnhancerreagent
• Gentleenoughtobeusedforsinglecellanalysis
• DownloadinvitroBHK-21transfectionprotocol:[PDF]
• Download BHK-21CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforBHK-21 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast86%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsBHK-21TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

BHK-21 CellLine:

Chronickidneyfailureisalossofkidneyfunctionoverthetimethatisconsideredasignificantpublichealthproblemworldwide.Developmentofnovelkidneytherapeuticsandinnovativetreatmentsoftendependsonpreclinicalstudiesthatcanhelptogaintheunderstandingoftheetiologyofthedisease.TheBHK-21celllinewasestablishedin1961from1-day-oldhamsterbyI.A.MacphersonandM.G.P.Stoker.Thiscelllineexhibitedfibroblastmorphologyandwastakenfromthekidneysofhealthygoldenhamster(Mesocricetusauratus).ThesecellsaresusceptIBLetohumanadenovirus25,poliovirus2,andreovirus3,amongothers.BHK-21cellsarebeneficialforstudyingmammaliankidneycellsBIOLOGy.

Adenovirusesbelongtoafamilyofvirusescharacterizedbythepresenceofnonenvelopeddouble-strandedDNAandtheabilitytoinfectawidevarietyofhumancells.Adenovirusespreferablytargetupperrespiratoryregionofthebody,andthesymptomsmanifestthemselvesintheformofhighlycontagiousdiseasessuchasconjunctivitis.Theyarealsoresponsibleformoresevereillnessessuchasmeningitis.Hamsterfibroblastswerefoundtobehighlypronetoinfectionadenoviruses,andthus,theBHK-21cellline.OvertimetheBHK-21hasproventobeareliabletransfectionhostduringpreclinicalstudiesinvolvingnotonlyadenovirusesbutotherpotentialpathogenssuchasreoviruses.AltogenBiosystemsprovidesbiodegradablepolymer-basedhighefficiencytransfectionreagentkitsthathaveexhibitedlowcytotoxicityandhighsiRNAuptakebyhostcells.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoBHK-21cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinBHK-21cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoBHK-21cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:

AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6175)
• 1.5ml(Catalog#6176)
• 1.5mlCRISPR(Catalog#2115)
• 8.0ml(Catalog#6177)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。