Description

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Transfection Reagent for MIA PaCa-2 Cells (Pancreatic Carcinoma Cells, CRL-1420)

• Proprietary cationic lipids formulation
• High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
• Effective and robust intracellular delivery
• Work in the presence of serum
• Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
• Download in vitro MiaPaCa2 transfection protocol: [PDF]
• Download MiaPaCa2 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for Mia-PaCa-2 cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 88% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems MiaPaCa-2 Transfection Protocol: [PDF]

Download MSDS: [PDF]

MIA PaCa-2 Cell Line:

Pancreatic adenocarcinoma is an aggressive malignancy that is often advanced at the time of initial diagnosis due to the lack of early symptoms and reliable screening tests as well as rapid progression of the disease. Established cell lines have proven to be a useful tool for investigating the epidemiology of pancreatic cancer.  The MIA PaCa-2 cell line was initially isolated by A. Yunis et al. in 1975 using pancreatic tumor cells of a 65-year-old Caucasian male with pancreatic carcinoma. The tumor was negative for an alkaline phosphatase stain and did not express high amounts of carcinoembryonic antigen. This is a hypotriploid cell line with floating rounded cells that display epithelial morphology. MIA PaCa-2 cells produce plasminogen activator and human colony-stimulating factor (CSF-1). The modal chromosome number for this cell line is 61, and a few normal chromosomes are absent. MIA PaCa-2 has a doubling time of roughly 40 hours and is an excellent host for biomedical research related to pancreatic cancer. Altogen Biosystems provides lipid-based transfection reagent kits for the MIA PaCa-2 cell line.

Data:

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the MIA Paca-2 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

Figure 2. Protein expression of Cyclophilin B in MIA PaCa-2 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into MIA PaCa-2 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems manufacturers preoptimized transfection kits for cancer research. Reagents and transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery into liver, pancreas, and kidney tissues), and in vivo biodistribution reagents (PEG-Liposome, Nanoparticle, Lipid, and Polymer-based kits). Optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, and RNA molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #6850)
• 1.5 ml (Catalog #6851)
• 1.5 ml CRISPR (Catalog #2174)
• 8.0 ml (Catalog #7064)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。