Description

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TransfectionReagentforNeuro-2aCells(NeuroblastomaCells,CCL-131)

• Proprietarycationiclipidsformulation
• Effectiveandrobustintracellulardelivery
• KitincludesTransfectionEnhancerreagent
• Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• DownloadinvitroNeuro2atransfectionprotocol:[PDF]
• Download Neuro2aCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforNeuro2a cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast71%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsNeuro2aTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Neuro-2aCellLine:

Neuroblastomaisthemostfrequentextracranialsolidtumorinchildren,androughlyhalfofthepatientsexperiencebonemetastasisaccompaniedbybonepain,leADIngtopoorprognosisforthoseaffected.TheNeuro-2acelllinewasestablishedfrombraintissueofanalbinohousemouse(Musmusculus)withneuroblastoma.Thesecellsexhibitneuronalandamoeboidstemcellmorphology,makingthemuniquelyusefulforscientificstudy.Also,theNeuro-2acelllineissusceptIBLetoherpessimplexvirus,humanpoliovirus1,andvesicularstomatitisvirus.Neuro-2acellshavebeenknowntocreatelargeamountsofmicrotubularprotein.Neuro-2acellsmakeforreliabletransfectionhostsduetotheirneuronalandamoeboidmorphology.AltogenBiosystemsoffersinvitrotransfectionreagentsdeliveringsatisfactoryresults,includingaliposomeformulationNeuro-2atransfectionreagentkitforneuroblastomacellsthatyieldshightransfectionefficiencyandisconsideredanappropriatehostforstudyingbraintumors.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRin Neuro2a cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

Figure2.ProteinexpressionofCyclophilinBinNeuro-2acells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoNeuro-2acellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6872)
• 1.5ml(Catalog#6873)
• 1.5mlCRISPR(Catalog#2184)
• 8.0ml(Catalog#7073)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。