Description

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TransfectionReagentforDI-TNC1Cells(RatBrainAstrocyteCells)

• Ananoparticle-basedliposomeformulation
• Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmiRNA
• Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
• Generatephysiologicallyrelevantdatayoucantrust
• EffectiveforplasmidDNA/siRNAco-transfection
• Easy-to-usetransfectionprotocolwithreproducIBLeresults
• Lowcytotoxicity
• DownloadDI-TNC1transfectionprotocol:[PDF]
• Download DI-TNC1CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforDI-TNC1cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast77%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsDI-TNC1TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

DI-TNC1CellLine:

Astrocytesarethemostabundanttypeofcellsinthecentralnervoussystemthatperformavarietyofdifferentfunctions,includingsupportofbrainmetabolism,theprimaryfocusofwhichisontherelationshipbetweenastrocytesandneurons. Astrocytesarecellsthatpromotethegrowthofneuronsbyprovidingnourishmentandsupport.DI-TNC1isderivedfromculturesofinterbraintissueofone-day-oldratsandcomprisesratbrainastrocytes.Culturedastrocyteshavebeenshowntopromoteneuriteoutgrowthbyproducingadhesionmoleculesfoundeitheronthecellsurfaceorintheextracellularmatrix.Thecellsretaincharacteristicsoftype1astrocytesincludingglialfibrillaryacidicprotein(GFAP)immunoreactivity.TheDI-TNC1celllineproducesalpha2macroglobulinsimilartoamountsfoundinprimaryastrocytesbutproducestransferrininmuchlesseramounts.ExaminationbyimmunostainingindicatedSV40Tantigenwasdetectedinthenucleiofover95percentofthecells.ResearchhasshownDI-TNC1cellsexhibitmanysimilaritiestoneonatalastrocytes,andthecelllineisutilizedinbiomedicalresearchforstudyingtheinteractionsbetweengliaandneurons,aswellasastrocytecellfunctionsrelatedtoenergymetabolism.TheDI-TNC1cellsresembleastrocytesfoundinhumaninfants.Neuriteoutgrowthisthedevelopmentofanyprojectionsinyoungnervecells.Attheearlystagesofgrowth,itishardtodiscernwhethertheprojectionsaredendriticoraxonic.ThecellsfromtheDI-TNC1celllinehaveexhibitedappreciableneuriteoutgrowth.AltogenBiosystemsmanufacturesananoparticle-basedtransfectionreagentkitfortheDI-TNC1ratastrocytecellline,anessentialtoolinfindingthecureforvariousbrain-relateddiseases.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheDITNC1cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

Figure2.ProteinexpressionofCyclophilinBinDI-TNC1cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoDI-TNC1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
• BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1747)
• 1.5ml(Catalog#1748)
• 1.5mlCRISPR(Catalog#2138)
• 8.0ml(Catalog#7036)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。