Description

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TransfectionReagentforBeta-TC-6Cells(PancreasTumor,CRL-11506)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroBeta-TC6transfectionprotocol:[PDF]
• Download Beta-TC-6CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforBeta-TC6 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast91%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsBeta-TC6TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Beta-TC-6CellLine:

Diabetesmellitusisadisorderthatoccurswhenthebodycannotmakegooduseoftheinsulinitproduces,orthepancreasfailstomakeenoughinsulin,whichresultsinhighbloodsugar.Insulinomaisarareneuroendocrinetumorthatformsinpancreaticisletcellsandismainlytreatedviasurgery.Therarityofinsulinomaposesachallengeinthedevelopmentofnoveltreatmentdrugsthatcanaddressit,astherearefewerparticipantsinclinicaltrials,whichmakescomprehensivepreclinicalstudiesespeciallycrucialinthequesttofindacureforit.Transfectionstudiescarriedoutonviablecancercelllinesarearobustandwidely-acceptedmethodforconductingpreclinicalresearchonalmostalltypesofcancer.TheBeta-TC-6celllinewasestablishedfrompancreatictumortissuefromatransgenicmouse(Musmusculus)sufferedfrominsulinoma.ThesecellsaretransgenicforSV40largeTantigenandexhibitepithelialcellmorphology(betacelltype).Theyhavebeenknowntoproduceglucagon,insulin,andsomatostatin,secretingmainlyinsulinspecificallyinresponsetothepresenceofglucose.Beta-TC-6cellsarebeneficialforbiomedicalresearchrelatedtodiabetes.

Data:

Figure1.GAPDHmRNAlevelswerequantifiedusingreal-timeRT-PCRintheBeta-TC6cellstransfectedwithsiRNAstargetingGAPDHornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHinBeta-TC-6cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoBeta-TC-6cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:

AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6150)
• 1.5ml(Catalog#6151)
• 1.5mlCRISPR(Catalog#2114)
• 8.0ml(Catalog#6152)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。