Description

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Transfection Reagent for HT-1080 Cells (Fibrosarcoma Cells, CCL-121)

• Two component formulation enhances lipid mediated transfection efficiency
• Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
• Kit includes Transfection Enhancer reagent and recommended transfection protocol
• Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
• Reproducible transfection results
• Works well for standard reverse transfection and high-throughput applications
• Download HT1080 transfection protocol: [PDF]
• Download HT1080 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for HT1080 cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems HT-1080 Transfection Protocol: [PDF]

Download MSDS: [PDF]

HT-1080 Cell Line:

Fibrosarcoma is a malignant tumor originating from fibrous connective tissue that mostly affects men in their 30s and 40s and involves immature proliferating fibroblasts. The HT-1080 cell line was originally established in 1972 from the tumorigenic connective tissue of a 35-year-old Caucasian male patient with fibrosarcoma. These cells exhibit an epithelial morphology and contain an activated N-ras oncogene. HT-1080 cells are susceptible to a variety of viruses, including polio, RD-114 feline virus, feline leukemia virus, and vesicular stomatitis virus. These cells are used for research related to cancer of connective tissues. A life science company Altogen Biosystems manufactures in vitro transfection kits for a vast array of cell lines, including the two-component lipid-based advanced formulation HT-1080 transfection reagent kit for human fibrosarcoma cells that yields high transfection efficiency of siRNA delivery.

Data:

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the HT-1080 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

Figure 2. Protein expression of GAPDH in HT-1080 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into HT-1080 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #6825)
• 1.5 ml (Catalog #6826)
• 1.5 ml CRISPR (Catalog #2156)
• 8.0 ml (Catalog #6827)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。