Description

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TransfectionReagentforHepG2Cells(HepatocellularCarcinomaCells,HB-8065)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• Effectiveandrobustintracellulardelivery
• KitincludesTransfectionEnhancerreagent
• Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroHepG2 transfectionprotocol:[PDF]
• Download HepG2CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforHepG2cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsHepG2TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

HepG2CellLine:

Livercancerishighlyaggressiveanddevastatingdiseasethatfeaturesseveralmaincharacteristics,includingahighrateofrecurrence,lowearlydetectionrateandineffectivetreatmentapproaches.Inadditiontobeingthefifthcommonmalignancy,itisconsideredthesecondprimarycauseofcancer-relatedmortalitiesworldwide,asstatedbyOncotarget,publishedinJuly2015.TheHepG2hepatomacelllineisimportantforthetoxicologicalassessmentofnovelcombinatorialdrugs.HepG2wasderivedfromthelivertissueofa15-year-oldmalewithdifferentiatedhepatocellularcarcinoma.Thesecellsareepithelialandhaveamodelchromosomenumberof55.HepG2cellssecreteavarietyofmajorplasmaproteins,includingalbumin,transferrin,alpha1-antitrypsin,alpha2-macroglobulin,andplasminogen(theyhavebeengrownsuccessfullyinlarge-scalecultivationsystems).Thecellsareresponsivetostimulationwithhumangrowthhormone.HepG2cellshavealsobeenshowntoberesistanttoG418.HepG2cellsandtheirderivativesareusedasamodelsystemforstudiesoflivermetabolismandtoxicityofxenobiotics,antigenotoxicandcogenotoxicagents,understandinghepatocarcinogenesis,thedetectionofcytoprotective,andfordrugtargetingstudies.Also,HepG2cellsarecommonlyemployedintrialswithbio-artificialliverdevices.Thiscelllineiswidelyusedinhumancancerresearchoftheliverandisessentialformanydifferenttypesofbiomedicalresearch.AltogenBiosystemsoffershighlyefficienttransfectionreagentkitsfortheHepG2hepatomacellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheHepG2cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofGAPDHinHepG2cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoHepG2cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

SelectedHepG2transfectionreagentproductcitations:

• JournalofMolecularEndocrinology2010.Identificationofproteinkinasesthatcontrolovarianhormone release…Sirotkinetal[PDF]
• FEBSLetters2009(583).MicroRNA125aanditsregulationofthep53tumorsuppressorgene…Zhangetal[PDF]
• CellularPhysiology2011.IdentificationofMicroRNAsControllingHumanOvarianCellProliferation …Sirotkinetal [PDF]

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• 2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• 201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• 201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
• BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
• 201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• 201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• 2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#2067)
• 1.5ml(Catalog#2068)
• 1.5mlCRISPR(Catalog#2154)
• 8.0ml(Catalog#2069)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。