Description

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TransfectionReagentforCalu-3Cells(LungCarcinomaCells,HTB-55)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• Effectiveandrobustintracellulardelivery
• KitincludesTransfectionEnhancerreagent
• Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroCalu-3 transfectionprotocol:[PDF]
• Download Calu-3CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforCalu-3cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Calu-3 CellLine:

Despitethehighlypreventablenatureoflungcancer,itremainsthesecondmostcommoncancerintheworldandhashighermortalityratesthancolon,breast,andprostatecancerscombined.LungcanceriscontinuouslylistedamongtheleADIngcausesofdeatharoundtheglobe,andfurtherresearchisneededtofindadefinitecure.Preclinicalresearchisanessentialelementforunderstandingtumorgrowthdynamicsanditsinteractionwiththeimmunesystem.Originallyderivedfromthelungtissueofa25-year-oldCaucasianmalewithadenocarcinoma,thetumOrigenicCalu-3celllinehasanepithelialcellmorphologyandisasuitablehostforstudiesrelatedtolungcanceraswellasforotherbiomedicalapplications.ThispatientwastreatedwithCytoxan,Bleomycin,andAdriamycinbeforeremovalofcells.AltogenBiosystemsprovidesefficientlipid-basedtransfectionreagentkitsfortheCalu-3lungcancercellline.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoCalu-3cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinCalu-3cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoCalu-3cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6401)
• 1.5ml(Catalog#6402)
• 1.5mlCRISPR(Catalog#2124)
• 8.0ml(Catalog#6403)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。