Description

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ElectroporationKitforKG-1Cells (MyelogenousLeukemiaCells,CCL-246)

•ElectroporationKitinclude:electroporationbuffer+ buffer-specificoptimizedelectroporationprotocols
•Pre-optimizedelectroporationprotocolsareprovidedfordeliveryofplasmids(DNA), siRNA,mRNA,andproteins
•Proprietaryelectroporationbuffer formulationmimicscellularcytoplasmcomposition(superiorelectroporeresealing)s
•CertifiedRNAse-andDNAse-free electroporationbuffer
• Effective electroporationdeliveryandhighcellviABIlity providesreproducIBLeelectroporationresults
•Highcellviabilityandsuperiorcellrecoveryrates
• Electroporationprotocolsare providedwithwiththepurchaseoftheElectroporation Kit
•DevelopedandmanufacturedbyAltogenBiosystems

ElectroporationBuffer:
4mLofelectroporationbuffer issufficientfor20 reactions usingstandard0.2cmelecrtoporationcuvette
30mLofelectroporationbuffer issufficientfor150 reactions usingstandard0.2cmelecrtoporationcuvette

ElectroporationEfficiency:
Electroporationkitprovides 70-85%delivery efficiencyofsiRNA.Electroporation efficiencywasdeterminedbyqRT-PCR.

Electroporation ProtocolandMSDS:
ElectroporationprotocolsareprovidedwiththepurchaseoftheElectroporationKit
DownloadMSDS:[PDF]

KG-1 CellLine:
TheKG-1celllinewasestablishedfromthebonemarrowofa59-year-oldCaucasianmalepatientwhosufferedfromacutemyelogenousleukemia(cancerofthebloodorbonemarrow).Thesecellsexhibitmyeloblastcellmorphology,andexpresstheHLAA30,A31,B35,andCw4antigens.KG-1cellsareEBNAnegative,andrespondwelltocolonystimulatingfactor(CSF).Thesecellsareusedforcancerbiomedicalresearch,especiallyrelatedtobonemarrow andleukemia.

Data:

AltogenBiosystems:

AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 4ml(Catalog#6575)
• 30ml(Catalog#6576)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。