Description

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ElectroporationKitforDaudiCells (Burkitt’sLymphomaCells,CCL-213)

• ElectroporationKitinclude:electroporationbuffer+ buffer-specificoptimizedelectroporationprotocols
• Pre-optimizedelectroporationprotocolsareprovidedfordeliveryofplasmids(DNA), siRNA,mRNA,andproteins
• Proprietaryelectroporationbuffer formulationmimicscellularcytoplasmcomposition(superiorelectroporeresealing)s
• CertifiedRNAse-andDNAse-free electroporationbuffer
•  Effective electroporationdeliveryandhighcellviABIlity providesreproducIBLeelectroporationresults
• Highcellviabilityandsuperiorcellrecoveryrates
•  Electroporationprotocolsare providedwithwiththepurchaseoftheElectroporation Kit
• DevelopedandmanufacturedbyAltogenBiosystems

ElectroporationBuffer:
4mLofelectroporationbuffer issufficientfor20 reactions usingstandard0.2cmelecrtoporationcuvette
30mLofelectroporationbuffer issufficientfor150 reactions usingstandard0.2cmelecrtoporationcuvette

ElectroporationEfficiency:
Electroporationkitprovides 70-85%delivery efficiencyofsiRNA.Electroporation efficiencywasdeterminedbyqRT-PCR.

Electroporation ProtocolandMSDS:
ElectroporationprotocolsareprovidedwiththepurchaseoftheElectroporationKit
DownloadMSDS:[PDF]

Daudi CellLine:
TheDaudicelllinewasoriginallyestablishedfromtheperipheralbloodofa16-year-oldmalepatientwhosufferedfromBurkitt’slymphoma,atypeofcanceroftheimmunesystem.Thesecellswereisolatedin1967byE.KleinandG.Klein.Theydon’texpressbeta-2-microglobulin,butarepositiveforEBNA,VCA,andsurfaceimmunoglobin(slg+).ThiscelllinehasbeentestedpositiveforEpstein-Barrvirus.Itcanbeusefulforstudiesofhowleukemiaforms,andpotentiallyforbiomedicalresearchrelatedtocertaintypesofcancer.

Data:

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• 2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]

AltogenBiosystems:

AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 4ml(Catalog#6561)
• 30ml(Catalog#6562)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。