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Transfection Reagent for SK-OV-3 Cells (Human Ovarian Carcinoma Cells, HTB-77)
Transfection Efficiency:
Reagent exhibits at least 74% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems SK-OV-3 Transfection Protocol: [PDF]
Download MSDS: [PDF]
SK-OV-3 Cell Line:
Ovarian cancer is considered the fifth primary cause of cancer deaths among women, accounting for more fatalities than any other type of cancer in females. Ovarian cancer mostly occurs in older Caucasian women over 60 years old and is one of the deadliest gynecological malignancy worldwide, as indicated by the American Cancer Society. Although ovarian cancer incidence rates have been consistently declining over the last decade, new treatment approaches are required to improve survival rates of those affected. The SK-OV-3 tumorigenic epithelial cell line was isolated in 1973 from the ovarian tissue of a 64-year-old Caucasian female with adenocarcinoma. SK-OV-3 cells have been known to be resistant to tumor necrosis, as well as several other cytotoxic drugs such as Adriamycin, cis-platinum, and diphtheria toxin. The SK-OV-3 cell line is hypodiploid and a suitable transfection host for ovarian cancer research. Altogen Biosystems offers pre-optimized lipid-based transfection kits for SK-OV-3 cells.
Data:
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the SKOV3 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Figure 2. Protein expression of Cyclophilin B in SK-OV-3 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into SK-OV-3 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
Altogen Biosystems:
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Company developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
Volume Options:
Altogen Biosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂、癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNA(mRNA,siRNA,shRNA,microRNA),蛋白质和小分子研究。
苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理,Altogen Biosystems华东代理,Altogen Biosystems华北代理,Altogen Biosystems全国代理,Altogen Biosystems华南代理。