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Transfection Reagent for SK-MEL-28 Cells (Melanoma Cells, HTB-72)
Transfection Efficiency:
Reagent exhibits at least 71% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems SK-MEL-28 Transfection Protocol: [PDF]
Download MSDS: [PDF]
SK-MEL-28 Cell Line:
Malignant melanoma is an invasive form of skin cancer that originates from the transformation of melanocytes. It is characterized by the highly aggressive nature and ability to metastasize to diverse distant sites. Human cell lines can provide a realistic prediction of drug responses in humans as well as information on safety and efficacy of drug candidates. The SK-MEL-28 cell line was established from the skin of a 51-year-old male patient with malignant melanoma. SK-Mel-28 cells exhibit polygonal cell morphology, and this is just one of many melanoma cell lines (also named SK-MEL). The modal chromosome number of 90 occurs nearly in 50% of cells. Also, the hypotetraploid SK-MEL-28 expresses wildtype N-Ras gene. This tumorigenic cell line could be helpful for skin cancer research as well as for other cell and molecular biology applications. Altogen Biosystems manufactures nanoparticle-based transfection kits for the SK-MEL-28 cell line.
Data:
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into SKMEL28 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in SK-MEL-28 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into SK-MEL-28 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
Altogen Biosystems:
Altogen Biosystems provides pre-optimized transfection kits and electroporation products for life science research. Transfection products are developed for individual cancer cell line and transfection protocols are optimized to enable high transfection efficiency of biomolecules. Altogen Biosystems developed in vivo delivery products for small animal research, mouse and rat targeted tissue delivery: liver targeted, pancreas targeted, kidney targeted, PEG-Liposome-, Nanoparticle-, Lipid-, and Polymer-based in vivo transfection kits. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of biomolecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
Volume Options:
Altogen Biosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂、癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNA(mRNA,siRNA,shRNA,microRNA),蛋白质和小分子研究。
苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理,Altogen Biosystems华东代理,Altogen Biosystems华北代理,Altogen Biosystems全国代理,Altogen Biosystems华南代理。