Description

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TransfectionReagentforPANC-1Cells(Non-endocrinePancreaticCancer)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroPANC-1transfectionprotocol:[PDF]
• DownloadPANC-1CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforPANC-1 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast85%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsPANC-1TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

PANC-1CellLine:

AccordingtoNature,pancreaticcancerhasa5-yearsurvivalrateof5%,makingthequestforareliablecureurgent.Pancreaticductaladenocarcinomaisthefourthprimarycauseofcancer-relatedfatalities,comprisingnearly90%ofallpancreaticmalignancies.PANC-1isawellcharacterizedandextensivelystudiedcelllinethatisfrequentlyemployedasinvitromodelforpancreaticductaladenocarcinomaresearch.ThePANC-1celllineplaysanessentialroleinpancreaticcancertherapeuticassays.ThePANC-1celllinewasestablishedfrompancreaticducttissueofa56-year-oldCaucasianmalewithepithelioidcarcinoma.PANCisshortforpancreas,referringtotheHumanpancreas,wherecancercanbefound.Also,thePANC-1celllineservesasasuitabletransfectionhost.ThegrowthofthesecellsisinhibitedbyL-asparaginase.

PANC-1cellsareusedasaninvitromodelofnon-endocrinepancreaticcancerfortumOrigenicitystudies.ThesecellspossessthetypeBphenotypeforglucose-6-phosphatedehydrogenaseG6PDandoverexpressheregulin/humanepidermalgrowthfactorreceptor2(HER2/neu)oncogene,whichispresentin60to70%ofhumanpancreaticcarcinomasbutareestrogenreceptor(ER)negative.PANCcellshavebeenshowntobenegativeforMUC4andpositiveforSmad4,aTGFbetasignalingcascadeproteininactivatedinhumangastrointestinalcancers.PANCcanbeinducedtodifferentiateintohormone-producingislet-likeclustersfollowingstimulationbytheFGF2growthfactor.ThePANC-1celllinehasanestimateddoublingtimeof52hourswithamodalchromosomenumberof63.Also,thesecellshaveGlucose-6-phosphatedehydrogenaseactivityoftheslowmobilityofBtype.AltogenBiosystemsprovidesproprietarycationicliposomeformulationthatdemonstratehighefficacytransfectionofPANC-1cells.TransfectionkitispreoptimizedfortheintracellulardeliveryofplasmidDNA,siRNA,andmRNAmoleculesintoPANC-1non-endocrinepancreaticcancercellline.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinthePANC-1cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).

Figure2.ProteinexpressionofCyclophilinBinPANC-1cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoPANC-1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#3225)
• 1.5ml(Catalog#3226)
• 1.5mlCRISPR(Catalog#2187)
• 8.0ml(Catalog#7076)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。