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主营:高分子化学,分子和细胞生物学,转染试剂
℡ 4000-520-616
℡ 4000-520-616
Altogen/NCI-N87 Transfection Kit (Gastric Carc. CRL-5822)/8.0 ml(Catalog #7072)
产品编号:8.0ml(Catalog#7072)
市  场 价:¥33140.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1657.00
品      牌: Altogen
公      司:Altogen Biosystems
公司分类:
Altogen/NCI-N87 Transfection Kit (Gastric Carc. CRL-5822)/8.0 ml(Catalog #7072)
商品介绍

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).


Transfection Reagent for NCI-N87 Cells (Gastric Carcinoma Cells, CRL-5822)

  • Two component formulation enhances lipid mediated transfection efficiency
  • Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
  • Kit includes Transfection Enhancer reagent and recommended transfection protocol
  • High transfection efficacy in the presence of serum
  • Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
  • Reproducible transfection results
  • Works well for standard reverse transfection and high-throughput applications
  • Download in vitro NCI-N87 transfection protocol: [PDF]
  • Download NCI-N87 CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for NCI-N87 cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 91% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems NCI-N87 Transfection Protocol: [PDF]

Download MSDS: [PDF]

NCI-N87 Cell Line:

According to the American Cancer Society (ACS), gastric cancer was the most common cause of cancer fatalities in the United States until the late 1930s. However, in less developed countries with a general lack of access to refrigerated food storage, it remains a high mortality disease. Human tumor cell lines have proven to be invaluable models for studying cancer causes and properties. The NCI-N87 epithelial cell line was established from the stomach tissue of a male patient with gastric carcinoma, also known as stomach cancer. NCI-N87 cells have been derived from a metastatic site located in the liver. Also, the NCI-N87 cell line has been tested positive for myc and ERBB2 oncogenes. NCI-N87 cells express carcinoembryonic antigen (CEA) and TAG 72 surface glycoproteins. They were also found to express receptors for muscarinic cholinergic agents. In addition to being used in stomach cancer research, the NCI-N87 cell line is highly utilized in transfection. Commercially available transfection reagent kits can be ordered from Altogen Biosystems, including a two-component formulation NCI-N87 transfection reagent for gastric carcinoma cells.

Data:

NCI-N87 Transfection Reagent

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into NCI-N87 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

NCI-N87-cells-transfection-protocol

Figure 2. Protein expression of Lamin A in NCI-N87 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into NCI-N87 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Request a Quote for
NCI-N87 Transfection Kit

Altogen Biosystems:
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6870)
  • 1.5 ml (Catalog #6871)
  • 1.5 ml CRISPR (Catalog #2183)
  • 8.0 ml (Catalog #7072)
品牌介绍


Altogen Biosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNAmRNAsiRNAshRNAmicroRNA),蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理,Altogen Biosystems华东代理Altogen Biosystems华北代理,Altogen Biosystems全国代理Altogen Biosystems华南代理

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