Description

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TransfectionReagentforMDCKCells(KidneyCells,CCL-34)

• Abiodegradablepolymerbasedtransfectionreagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• AchieverobustsiRNAuptakefordependablegenesilencing
• Effectivetransfectionunderconditionsofupto40%serum
• TransfectionkitincludesTransfectionEnhancerreagent
• DownloadMDCKtransfectionprotocol:[PDF]
• Download MDCKCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforMDCKcellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsMDCKTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

MDCKCellLine:

TheepithelialcelllineMADIn-DarbyCanineKidney(MDCK)wasderivedfromthekidneycellsofanadultcockerspaniel(Canisfamiliaris)in1958andispositivelytestedforkeratinbyimmunoperoxidasestaining.Thisisamodelmammaliancelllineextensivelyemployedinbiomedicalresearch. MDCKcellsareusefulforprocessingofbeta-amyloidprecursorproteinanditsproducts.MDCKcellsaresusceptIBLetoviralinfectionbutmakegoodtransfectionhost.Thesecellscouldbehelpfulinstudyingkidneycellsfunctionandgeneexpressionforbiomedicalresearchpurposes.FurThermore,theMDCKcelllineiswidelyusedininfluenzastudies.MDCKcellsarehyperdiploidwithabi-modalchromosomenumberdistribution.MostspreadscompriseonenormalXchromosome,buttherearenoconsistentidentifiableMarkerchromosomes.AltogenBiosystemsproducespolymer-basedtransfectionreagentkitsfortheMDCKcellline.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoMDCKcellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinMDCKcells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoMDCKcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6845)
• 1.5ml(Catalog#6846)
• 1.5mlCRISPR(Catalog#2171)
• 8.0ml(Catalog#7061)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。