Description

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TransfectionReagentfor KBCells(MouthEpidermalCarcinomaCells)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• Effectiveandrobustintracellulardelivery
• KitincludesTransfectionEnhancerreagent
• Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroKB transfectionprotocol:[PDF]
• Download KBCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforKB cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsKBTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

KB CellLine:

TheKBcelllinewasfirstbelievedtobeestablishedfromaremovalofhumanmouthepidermalcarcinomaofthenasopharynx,atypeofthroatcancer.KBcellsexhibitanepithelialcellmorphologyandareknowntoproducekeratin.ManymedicalapplicationsexistforKBcellline.However,recenttestinghasidentifiedHeLacelllineMarkerchromosomesintheKBcellline,andthroughDNAfingerprinting,KBcellsarenowthoughttohavebeenproducedduetoHeLacellcontamination.FurThermore,KBcellscontainhumanpapillomavirus18sequences,justliketheHeLacelllinedoes,andarepositiveforkeratinbyimmunoperoxidasestaining.TheKBlineisalsousedasanassayforantineoplasticagents.AnendoribonucleasewhichcleavestRNAprecursormoleculeshasbeenpartiallypurifiedfromhumanKBtissueculturecells.KBcellshavebeenusedbytheNationalCancerInstituteasanantitumorassayforscreeningplantextracts.

NowKBisknowntobeasublineoftheKERATIN-formingtumorcelllineHeLathatwasestablishedin1951usingcervicaladenocarcinomacellstakenfromHenriettaLacks,a31-year-oldworking-classAfrican-Americanwomanwhodiedofcervicalcancer.TheHeLacelllineisthefirsthumancelllinesuccessfulinvitroinacellculturethatisalsoconsideredan“immortal”cellline.HeLacellshavereceivedmediaattentionbecauseofthecontroversyoverpatient’srightsasthecellsweretakenwithoutthepermissionorknowledgeofthepatientandherfamily.TheHeLacelllinehasplayedavitalroleinthedevelopmentofapoliovaccineandcontributedtothetelomerasediscovery.AltogenBiosystemsprovidesreliabletransfectionreagentkitsfortheKBcellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheKBcellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHinKBcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoKBcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsmanufacturerspreoptimizedtransfectionkitsforcancerresearch.Reagentsandtransfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliveryintoliver,pancreas,andkidneytissues),andinvivobiodistributionreagents(PEG-Liposome,Nanoparticle,Lipid,andPolymer-basedkits).Optimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,andRNAmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1781)
• 1.5ml(Catalog#1782)
• 1.5mlCRISPR(Catalog#2163)
• 8.0ml(Catalog#7052)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。