Description

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TransfectionReagentforHUH-7Cells(LiverCancerCells)

• Abiodegradablepolymerbasedtransfectionreagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• Gentleenoughtobeusedforsinglecellanalysis
• DownloadinvitroHuh7transfectionprotocol:[PDF]
• Download Huh7CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforHuh7 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast88%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsHuh-7TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

HUH-7 CellLine:

AccordingtotheAmericanCancerSociety,livercancerhasamuchbetterprognosisforcompleteremissionandsurvival,ifdiagnosedearly.FurThermore,livercancercaughtearlyhasa50%5-yearsurvivalrate,versusanonly3%survivalrateifitisdetectedlateandhasspreadtootherorgans.TheHuH-7hepatomacelllineconsistsofwell-differentiatedtumOrigeniclivercellsthatareusefulforcarryingouttransfectionstudies.TheHuH-7celllinewasderivedfromalivertumorofa57-year-oldJapanesemalepatientin1982.HuH-7isatypeofimmortalcellline,ashepatomacells,theyexhibitanepithelialcellmorphology.InadditiontobeingasuitabletransfectionhostformultiplecellularandmolecularBIOLOGyapplications,thiscelllineisextensivelyemployedinlivercancerresearch.Commonformsoflivercancerareusuallycausedbyviralhepatitis(BorC)infectionorcirrhosis.AltogenBiosystemsofferspolymer-basedbiodegradabletransfectionreagentsfortheHuH-7hepatomacellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheHuh-7cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHinHUH-7cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoHUH-7cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1775)
• 1.5ml(Catalog#1776)
• 1.5mlCRISPR(Catalog#2158)
• 8.0ml(Catalog#1777)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。