Description

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TransfectionReagentforHEK-293Cells(EpithelialKidneyCells, CRL-1573)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadHEK293transfectionprotocol:[PDF]
• Download HEK293CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforHEK293 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsHEK-293TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

HEK-293CellLine:

Humanembryonickidneycellshavehightransfectionefficiencyandarecommonlyusedincancerresearchaswellasforpharmaceuticalandbiomedicalpurposes.HEK-293humanepithelialkidneycellswereoriginallyderivedin1977fromhumanembryonickidneycellstransformedwithshearedadenovirus5DNAgrownintissueculture.Thetransformationincorporatedapproximately4.5kilobasesfromtheviralgenomeintohumanchromosome19oftheHEKcells. HEKhasbeendescribedasbeingacellulartesttube.Thesecellsweredevelopedfromembryonickidneycells,andareusefulinavarietyofmolecularandBIOLOGicalresearch,manyofwhichhavemedicalapplications.Inadditiontobeingreliablehostsfortransfection,HEK-293cellsareparticularlyhelpfulfortheproductionoftherapeuticproteinsaswellasvirusesforgenetherapy.AltogenBiosystemsofferspre-optimizedadvancedformulationoflipid-basedtransfectionreagentkitsforhumanepithelialkidneycellsthatyieldatleast90%transfectionefficiency,asverifiedbyqRT-PCR.

Data:

Figure1.SiRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoHEK-293cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofLaminA/CinHEK-293cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoHEK-293cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1763)
• 1.5ml(Catalog#1764)
• 1.5mlCRISPR(Catalog#2149)
• 8.0ml(Catalog#7046)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。