Description

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TransfectionReagentforHCC1937Cells(BreastCarcinomaCells,CRL-2336)

• Abiodegradablepolymerbasedtrasfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• AchieverobustsiRNAuptakefordependablegenesilencing
• Effectivetransfectionunderconditionsofupto40%serum
• TransfectionkitincludesTransfectionEnhancerreagent
• DownloadinvitroHCC1937 transfectionprotocol:[PDF]
• Download HCC1937CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforHCC1937cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast84%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsHCC1937TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

HCC1937CellLine:

Breastcancerremainsthesecondprimarycauseofcancerdeathsinfemalesworldwide,withmorethan40,000fatalitiesannuallyintheUnitedStatesalone.However,breastcancerdeathratesinolderwomenhavebeendecreasingforthelastdecadeprimarilyduetosignificantadvancesinbreastcancermanagementanditsearlydiagnosis,asstatedbytheAmericanCancerSociety(ACS).Ductalcarcinomacomprisesabout80%ofallbreastcancerdiagnoses.Itbeginsinthemilkductsandspreadstosurroundingbreasttissue.Despitenoveltreatmentoptions,breastcancerremainsamongthetopcausesoffemaledeaths,globally.Researchcarriedoutusingtransfectioncanleadtorevelationsthatcouldhelpdevisecombinatorialtreatmentstocurebreastcancer.TheHCC1937celllineisderivedinitiallyfromthemammaryglandofa23-year-oldCaucasianfemalewithbreastcarcinomathatisassociatedwithgeneticpredisposition.HCC1937wasestablishedfromaprimaryductalTNMStageIIB,grade3carcinomain1996.Thistypeofbreastcancerisbelievedtohavegeneticlinksbecauseinmanycasesmultiplefamilymemberswillbeaffected.Thiscelllinecouldbeusefulforthestudyofdifferenttypesofbreastcancer.HCC1937’smodalchromosomenumberis100.Eachcelldoesn’tpossessafullyintactXorYchromosome.AltogenBiosystemsmanufacturespre-optimizedpolymer-basedbiodegradabletransfectionreagentkits.ThereagenthasexhibitedhightransfectionefficiencyfortheHCC1937breastcarcinomacellline.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoHCC1937 cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinHCC1937cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoHCC1937cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsmanufacturerspreoptimizedtransfectionkitsforcancerresearch.Reagentsandtransfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliveryintoliver,pancreas,andkidneytissues),andinvivobiodistributionreagents(PEG-Liposome,Nanoparticle,Lipid,andPolymer-basedkits).Optimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,andRNAmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6807)
• 1.5ml(Catalog#6808)
• 1.5mlCRISPR(Catalog#2145)
• 8.0ml(Catalog#7042)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。