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主营:高分子化学,分子和细胞生物学,转染试剂
℡ 4000-520-616
℡ 4000-520-616
Altogen/Detroit551 Transfection Kit (Embryonic Fibroblast Cells)/8.0 ml(Catalog #7035)
产品编号:8.0ml(Catalog#7035)
市  场 价:¥31340.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1567.00
品      牌: Altogen
公      司:Altogen Biosystems
公司分类:
Altogen/Detroit551 Transfection Kit (Embryonic Fibroblast Cells)/8.0 ml(Catalog #7035)
商品介绍

Description

ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).

Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).


TransfectionReagentforDetroit-551Cells(EmbryonicFibroblastCells,CCL-110)

  • Abiodegradablepolymerbasedtransfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
  • HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
  • AchieverobustsiRNAuptakefordependablegenesilencing
  • Effectivetransfectionunderconditionsofupto40%serum
  • Gentleenoughtobeusedforsinglecellanalysis
  • DownloadDetroit-551 transfectionprotocol:[PDF]
  • DownloadDetroit-551_CRISPR/Cas9transfectionprotocol:[PDF]
  • DownloadPowerPointpresentationforDetroit-551cellstransfectionkit:[PPT]
  • DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast78%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsDetroit-551TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Detroit-551CellLine:

FibroblastsarethemostcommonconnectivetissuecellspresentinthebodythatareresponsIBLeformakingupthestructuralframework.EmbryonicstemcellsarecommonlyemployedforcellandmolecularBIOLOGyresearchandareimperativeforthepropergrowthofstemcells.TheDetroit-551celllinewasinitiallyestablishedin1965usingskintissuetakenfromtherelativelyhealthyfetusofaCaucasianfemale.Thesecellsexhibitafibroblastmorphologyandcelltypeandarefrequentlyusedasfeedercellsforhumanembryonicstemcellresearch.Also,thiscelllineisanadequatehostforstudiesrelatedtomammalianembryonictissue.DuetotheirabilitytohavetheirgrowthenhancedbythepresenceofTNF(tumornecrosisfactor)alpha,Detroit-551cellsarewidelyusedinbiomedicalresearchapplications.ThiscelllinecanbetransformedbySV40,andhaveaLifespanof~25passages.Detroit-551cellsserveascontrolsinthestudyofmutatedcellsastheyarenormal,buttheycanalsobequicklytransformedbyviruses.AltogenBiosystemsprovidespolymer-basedbiodegradabletransfectionreagentkitsfortheDetroit-551fibroblastcellline.Thetransfectionreagenthasexhibitedhighefficiencyandhighsensitivity.

Data:

Detroit551 Transfection Reagent

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoDetroit-551cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Detroit551-cells-transfection-protocol

Figure2.ProteinexpressionofLaminA/CinDetroit-551cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoDetroit-551cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

RequestaQuotefor
Detroit551TransfectionKit

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

  • 0.5ml(Catalog#6801)
  • 1.5ml(Catalog#6802)
  • 1.5mlCRISPR(Catalog#2137)
  • 8.0ml(Catalog#7035)
品牌介绍


Altogen Biosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNAmRNAsiRNAshRNAmicroRNA),蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理,Altogen Biosystems华东代理Altogen Biosystems华北代理,Altogen Biosystems全国代理Altogen Biosystems华南代理

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