Description

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TransfectionReagentforD-407Cells(RetinalPigmentEpithelialCells)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• KitincludesTransfectionEnhancerreagent
• Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroD407 transfectionprotocol:[PDF]
• Download D-407CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforD407 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsD-407 TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

D-407CellLine:

Despiterecentbreakthroughsinthetreatmentandpreventionofeyediseasesthatleadtoblindness,therearestillmanycausesofvisualimpairmentforwhichthereisnotreatment.Preclinicalstudiesoftheretinalpigmentepithelium(RPE)canhelptoovercomeobstaclesinfindingnewtreatmentsforage-relatedmaculardegeneration(AMD),theprimarycauseofblindnessintheworld.Retinalpigmentepithelium(RPE)isalayerofcellsintheeyethatprovidesnourishmenttoretinalcellsandperformsavarietyoffunctionsrelatedtotheupkeepofnormalvision.OvertimetheRPEdegeneratesandleadstoavarietyofvisiondisorders.TheD-407celllinewasderivedfromhumanRPEcellsandisavaluableresearchtoolinthestudyofvisionimpairment.Thiscelllineisusefulinstudiesofhumaneyecells,furtherinvestigationofthiscelllinecouldproducepotentialmedicaltreatmentsforthevisuallyimpaired. D-407cellsexhibitphagocyticactivityandareusedwidelyinmolecularandcellBIOLOGyresearch.ResearchcarriedoutviatransfectionontheD-407RPEcelllinecouldleadtothedevelopmentoftreatmentsthatcouldhelprestorevisioninpatients.AltogenBiosystemsprovideslipid-basedtransfectionreagentkitsfortheD-407retinalpigmentepitheliumcelllinethathasproducedconsistentresultsintheestablishmentofstablecelllines.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheD-407cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.

Figure2.ProteinexpressionofGAPDHinD-407cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoD-407cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1743)
• 1.5ml(Catalog#1744)
• 1.5mlCRISPR(Catalog#2135)
• 8.0ml(Catalog#7033)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。