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主营:高分子化学,分子和细胞生物学,转染试剂
℡ 4000-520-616
℡ 4000-520-616
Altogen/CT26.WT Transfection Reagent (Colon Carcinoma)/8.0 ml(Catalog #6456)
产品编号:8.0ml(Catalog#6456)
市  场 价:¥31880.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1594.00
品      牌: Altogen
公      司:Altogen Biosystems
公司分类:
Altogen/CT26.WT Transfection Reagent (Colon Carcinoma)/8.0 ml(Catalog #6456)
商品介绍

Description

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TransfectionReagentforCT26.WTCells(ColonCarcinomaCells,CRL-2638)

  • Proprietarycationiclipidsformulation
  • HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
  • Effectiveandrobustintracellulardelivery
  • Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
  • DownloadinvitroCT26WTtransfectionprotocol:[PDF]
  • Download CT26.WTCRISPR/Cas9transfectionprotocol:[PDF]
  • DownloadPowerPointpresentationforCT26WTcellstransfectionkit:[PPT]
  • DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast92%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsCT26WTTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

CT26.WTCellLine:

ColoncanceristhefourthmostcommoncancerdetectedintheUnitedStates.InadditiontobeingresponsIBLeforover95,000newcasesannually,ithasanincreasedincidencerateinpatientsyoungerthanage50,aspertheColonCancerAlliance(CCA).TheCT26.WTcoloncancercelllinewasderivedfromBALB/cmicethatdevelopedaggressivecoloncarcinomaasaresultofchemicallyinducedmutationandclonedwiththeretroviralvectorLXSN,containingthelacZgene.CT26.WTisahighlytumOrigeniccellline,whichiscommonlyemployedinthedrugdevelopmentforcoloncancer.Also,CT26.WTcellsareoftenusedasamodelforthetestingofimmunotherapyprotocols,aswellastransfectionhostcelllineforresearchstudiesontheimmuneresponse.AltogenBiosystemsprovideslipid-basedhigh-efficiencytransfectionreagentkitsfortheCT26.WTcelllinethathavedemonstratedconsistentresultsandlowcytotoxicity.

Data:

CT26WT Transfection Reagent

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinCT26WTcellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

CT26WT-cells-transfection-protocol

Figure2.ProteinexpressionofCyclophilinBinCT26.WTcells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoCT26.WTcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

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CT26.WTTransfectionReagent

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

  • 0.5ml(Catalog#6454)
  • 1.5ml(Catalog#6455)
  • 1.5mlCRISPR(Catalog#2134)
  • 8.0ml(Catalog#6456)
品牌介绍


Altogen Biosystems是一家开发和制造用于生命科学研究,药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化,可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外(癌细胞系)和体内(动物组织靶向试剂癌细胞系)递送货物分子,包括质粒DNA,各种类型的RNAmRNAsiRNAshRNAmicroRNA),蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理,Altogen Biosystems华东代理Altogen Biosystems华北代理,Altogen Biosystems全国代理Altogen Biosystems华南代理

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