Description

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TransfectionReagentfor CHO-K1Cells(OvarianAdenocarcinomaCells,CRL-9618)

• Aliposome-based lipidformulation
• TransfectionEnhancerreagentprovidedwiththekit
• Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
• Generatephysiologicallyrelevantdatayoucantrust
• EffectiveforplasmidDNA/siRNAco-transfection
• Easy-to-usetransfectionprotocolwithreproducIBLeresults
• Lowcytotoxicity
• DownloadinvitroCHO-K1transfectionprotocol:[PDF]
• Download CHO-K1CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforCHO-K1cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast92%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsCHO-K1TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

CHO-K1 CellLine:

Hamsters,likemice,makeforgoodresearchmodels,andtheChineseHamsterOvary(CHO)celllinehaslongbeenusedintheinvestigationofnewdrugs.TheCHO-K1celllineisisolatedfromasubcloneoftheCHOcelllinethatwasderivedfromovarycellsofanadultChinesehamster(cricetulusgriseus)in1957.ThecellsfromtheCHO-K1celllineareresistanttoanumberofviruses,includingpoliovirus2,ButtonWillowvirus,andModocvirus.Also,CHO-K1cellsareespeciallyusefulforthesynthesisoftherapeuticproteinsinadditiontotheroletheyplayinmedicalandbiochemicalresearch.Thiscelllinerequiresthepresenceofprolineforgrowthbutmakesforagoodtransfectionhost.ThesecellsareinvaluableinavarietyofbiomedicalresearchapplicationsrelatedtomolecularandcellBIOLOGy.AltogenBiosystemsdesignsanadvancedformulationofaliposome-basedlipidCHO-K1transfectionreagentfortheCHO-K1cellline,whichhasexhibited~92%transfectionefficiencyandlowcytotoxicity.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoCHO-K1cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinCHO-K1cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoCHO-K1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6450)
• 1.5ml(Catalog#6451)
• 1.5mlCRISPR(Catalog#2129)
• 8.0ml(Catalog#7030)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。