Description

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TransfectionReagentforCFPEo(HTRpC)Cells(TracheaEpitheliumCells)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• Effectiveandrobustintracellulardelivery
• KitincludesTransfectionEnhancerreagent
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroCFPEotransfectionprotocol:[PDF]
• Download CFPEoCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforCFPEocellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsCFPEoTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

CFPEo(HTRpC)CellLine:

Approximately14%ofallnewlydiagnosedcancersarelungcancersthatmainlyoccurinolderpeople,withanaveragepatientagebeingaround70yearsold,accordingtotheAmericanCancerSociety(ACS).TheCFPEocelllinewasestablishedusinghumantracheaepitheliumcells(alsoknownasHTEpCCellLine).HumanTracheaEpitheliumCells(HTEpC)areresponsIBLeforkeepingtheairwayscleanandprovidingproperlubricationforairpassage.HTEpCareusuallyimpactedintheeventofarespiratorydisorderandhencemakeforappropriateinvestigativeandtoxicologicalresearchtools.Thesetypesofcellsintheairwaysareresponsibleformaintaininghumidityaswellascleaningtherespiratorytract.Inadditiontobeinganimportanttargetfordrugs,toxins,andcarcinogens,CFPEocellsarebeneficialcellculturemodelsinbiomedicalresearchfortreatmentsofasthma,viralinfection,fibroticlungdisease,lungcancer,andcysticfibrosis.Consequently,CFPEoareeffectiveforinvestigatingthefunctionandpathologyoftherespiratorysystem.Theyarealsousedindruguptakeexperiments,toxicologicalandwoundhealingresearch,oncology,andcelldifferentiation.InvestigationsincludestudyingtheregulationofintracellularpH,thestimulanteffectofIL-1bonairwayepithelialcellgrowth,andhowhumanrhinovirusinfectionaffectsupregulation,orhastening,ofICAM-1expressiononepithelialcells.AltogenBiosystemsprovidestheCFPEo(HTRpC)transfectionreagentkitforhumantracheaepitheliumcellsthatyieldshightransfectionefficiency.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheCFPEOcellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

Figure2.ProteinexpressionofCyclophilinBinCFPEocells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoCFPEocellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Companydevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits). Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1729)
• 1.5ml(Catalog#1730)
• 1.5mlCRISPR(Catalog#2127)
• 8.0ml(Catalog#1731)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。