Description

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TransfectionReagentfor Calu-6Cells(LungCarcinomaCell,HTB-56)

• Liposomeformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• DownloadinvitroCalu-6transfectionprotocol:[PDF]
• Download Calu-6CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforCalu-6cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsCalu-6TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Calu-6 CellLine:

LungcancerisconsideredtheleADIngcauseofcancerdeathworldwideamongbothwomenandmen,mainlyoccurringinolderpeople,withanaveragepatientageof70yearsandlessthan2%ofalldiagnosedpatientsbeingyoungerthan45,pertheAmericanCancerSociety(ACS).Moreover,itisconsideredthemostpreventablecancer,affectingsmokers15to30timesmoreoftenthanpeoplewhodonotsmoke.TheCalu-6epithelialcelllinewasderivedfromtissueofa61-year-oldCaucasianfemalepatientwithanaplasticcarcinoma(lungcancer).Anaplasticcarcinomaisaconditioninwhichcellsrapidlyproliferate,butlosetheiroriginaldifferentiation,leadingtohighmalignancy.ThemodalchromosomenumberintheCalu-6celllineis59,andthesecellsarehighlyutilizedininvestigativeresearch.Calu-6cellshavebeenusedtobetterunderstandtherelationshipbetweenantimycinA(AMA)andlungcancercells.Ithasbeensuggestedinstudiesthatchangesofreactiveoxygenspecies(ROS)andglutathione(GSH)affectapoptosisinAMA-treatedCalu-6cells.Also,usingAMAasamitochondrialelectrontransportinhibitordecreasedthegrowthofCalu-6cellsbyinducingaG1arrestofthecellcycleandapoptosis.Tiron,aROSscavenger,protectshumanlungcancerCalu-6cellsagainstantimycinA-inducedcelldeath.TheCalu-6celllinehasalsobeenemployedinresearchofthemechanismunderlyingpressuretransductionoftherenin-angiotensinsystem.AltogenBiosystemsprovidespre-optimizedtransfectionreagentkitsfortheCalu-6anaplasticlungcancercelllinethatyieldaround90%transfectionefficiency,asperqRT-PCR.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheCalu-6cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofGAPDHinCalu-6cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoCalu-6cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

SelectedCalu6 transfectionreagentcitationreferences:

• ClinLungCancer.2015Nov;16(6):e121-9.miRProfilingIdentifiesCyclin-DependentKinase6DownregulationasaPotentialMechanismofAcquiredCisplatinResistanceinNon-Small-CellLungCarcinoma.Baretal[PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]

AltogenBiosystems:
AltogenBiosystemsmanufacturerspreoptimizedtransfectionkitsforcancerresearch.Reagentsandtransfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliveryintoliver,pancreas,andkidneytissues),andinvivobiodistributionreagents(PEG-Liposome,Nanoparticle,Lipid,andPolymer-basedkits).Optimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,andRNAmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.OurBIOLOGyCROservices include:Xenograftservices(over60in-housevalidatedxenograftmodels),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1727)
• 1.5ml(Catalog#1728)
• 1.5mlCRISPR(Catalog#2125)
• 8.0ml(Catalog#7027)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。