Description

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TransfectionReagentforC2C12Cells(MouseMyoblastCells,CRL-1772)

• Lipid-basedtransfection reagent
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroC2C12transfectionprotocol:[PDF]
• Download C2C12CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforC2C12 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast88%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsC2C12TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

C2C12CellLine:

Myocytesarelongtubularcellsthatarisefrommyoblastsandmakeupmusclefibersinhumanbeings.SubcloningisthetargetedtransferofaspecificDNAsequencefromtheparentvectortothetargetone.TheC2C12mousemyoblastcelllineisamuscletissuecelllinethatwasderivedfromasubcloneofamouse(Musmusculus)cellline.C2C12isamuscletissuecellline,whichexhibitsmyoblastmorphology.Thiscelllineactsasagoodtransfectionmodel,butcanrapidlydifferentiateintocontractilemyotubes,producingmuscle-specificproteins.C2C12hasbeentestednegativeformousepox(ectromeliavirus).ThesecellscouldbeusedforbiomedicalresearchandcellBIOLOGy,especiallyofmammalianmyoblastcells.Owingtoitsabilityforrapiddifferentiationthatcanbeeasilymodulatedbytreatmentwithproteins,theC2C12celllinehasproventobeareliabletransfectionhostforthestudyofmusclecells.AltogenBiosystemsprovidespre-optimizedlipid-basedtransfectionreagentkits,whichhavedemonstratedhightransfectionefficiencyverifiedbyqRT-PCR,fortheC2C12mousemyoblastcellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheC2C12cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHinC2C12cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoC2C12cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]

AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Transfectionreagentsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedformaximumdeliveryefficiency.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6343)
• 1.5ml(Catalog#6344)
• 1.5mlCRISPR(Catalog#2120)
• 8.0ml(Catalog#7025)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。