Description

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TransfectionReagentforBMSandBMS2 Cells(BoneMarrowCells)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• Effectiveandrobustintracellulardelivery
• KitincludesTransfectionEnhancerreagent
• Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• DownloadinvitroBMS2 transfectionprotocol:[PDF]
• Download BMS2CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforBMS-2cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsBMS2TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

BMS2 CellLine:

AspertheLeukemiaandLymphomaSociety,approximately160peopledieofleukemiaeveryday,intheUnitedStates.Althoughnumeroustreatmentoptionsareavailableforpeoplewithbonemarrowcancer(multiplemyeloma),remissionisnotalwaysacure,andnewtherapiesarecriticalforimprovingthesurvivalrateofmultiplemyelomapatients.Preclinicalstudiesofbonemarrowcanhelpresearchersfindnoveltreatmentsforpatientswithbonemarrowdisorders.Newredbloodcells(RBCs)aresynthesizedinaprocesscalledhematopoiesisinsidearegioncalledthebonemarrowoflongbonessuchasthefemur,locatedinthethigh.Bonemarrowistheprimarytargetinvariousformsofleukemia.FurThermore,bonemarrowishighlysusceptIBLetopreventativerADIationtherapyasitcausesadisturbanceinRBCproduction,whichcouldleadtoimmunodeficiencyandonlyworsenthegrowthofcancer.TheBoneMarrowStromal(BMS)celllinewasestablishedfrommammaliantissueandhasproventobeusefulincellandmolecularBIOLOGyresearchthatemploystransfectiontoinvestigatethesecellsthoroughly.Bonemarrowistheflexiblematerialintheinteriorofmostbones.Inlargebones,itisthesourceofnewbloodcells.Thestromaisalltissuenotdirectlyinvolvedmakingbloodcells.Thesepropertiesmakethesetypeofcellsvaluableformanykindsofbiomolecularstudies.Also,thiscelllineisanadequatehostforstudyingskincancerandotherformsofcancer.High-efficiencytransfectionreagentsconsiderablyimprovethequalityofthetransfectionprocedure.AltogenBiosystemsprovideslipid-basedtransfectionreagentkitsthathaveexhibitedhighefficiencyinestablishingstableBMSandBMS2celllines.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheBMS2cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).

Figure2.ProteinexpressionofCyclophilinBinBMS2cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoBMS2cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1721)
• 1.5ml(Catalog#1722)
• 1.5mlCRISPR(Catalog#2117)
• 8.0ml(Catalog#7024)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。