Description

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TransfectionReagentforB16-F10Cells(MouseMelanomaCells,CRL-6475)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroB16-F10transfectionprotocol:[PDF]
• Download B16-F10CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforB16-F10 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

Reagentexhibitsatleast83%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsB16-F10TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

B16-F10CellLine:

MalignantmelanomaisahighlymetastaticformofcanceraswellasthesixthmostcommoncancerintheUnitedStates.Preclinicalstudiesofmelanomacanhelpscientiststogainabetterunderstandingofdrugresponsesinhumansandfindnoveltherapeuticsforthedisease.Micehavebeenusedasmammalianresearchmodelssincethe16ᵗʰcentury.Theyareusefulinpreclinicalstudiesastheircellsaregoodpredictorsofapotentialdrug’sperformance.TheB16-F10melanomacelllines,firstdiscoveredin1954,arederivedfromnaturallyoccurringtumorsofamouse(Musmusculus)sufferedfrommelanoma.Thiscelllineexhibitsamorphologythatisamixtureofepithelialandspindle-shapedcells.TheB16-F10celllinecouldbehelpfulformolecularandcellBIOLOGyexperimentalresearch.FurThermore,theB16-F10melanomacelllineisasuitablehostforcarryingouttransfectionduringinvestigativeandpreclinicaloncologicalresearch.AltogenBiosystemscreateshigh-efficiencycustomizedtransfectionreagentkitsforB16-F10mousemelanomacells.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinB16-F10 cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

Figure2.ProteinexpressionofCyclophilinBinB16-F10 cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoB16-F10cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:

AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6135)
• 1.5ml(Catalog#6136)
• 1.5mlCRISPR(Catalog#2111)
• 8.0ml(Catalog#6137)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。