Description

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TransfectionReagentforASMCCells(AorticSmoothMuscleCells)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroASMC transfectionprotocol:[PDF]
• Download ASMCCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforASMCcellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsASMCTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

ASMC CellLine:

AccordingtoAmericanHeartAssociation,nearly92.1millionAmericanssufferfromacardiovasculardisease(CVD)ofsomesort.PreclinicalresearchiscriticalinunderstandinghowthearteriesrespondtovariousBIOLOGicalandchemicalfactors,andhencedeviseinnovativemethodstoaddressCVDs.HumanAorticSmoothMuscleCellsarederivedfromtunicaintimaandtunicamediaofplaque-freeaortatissue.Thetunicaintimaistheinnerlayerofthearterycomprisedofalining,anetworkofconnectivetissue,andalayerofelasticfibers;thetunicamedia,ormiddlecoat,iscomposedmostlyofsmoothmusclecellsandelasticfibersarrangedinspirallayers.ArterialSmoothMuscleCell(ASMC)linehasbeenusefulinthestudyofatherosclerosis,avasculardisordercharacterizedbylossofflexibilityinbloodvesselsduetotheaccumulationofdeadwhitebloodcells,cholesterol,andcrystallizedsalts,whichleadstonarrowingofarteriesandmaycausefurtherproblemssucharrhythmiaandstrokes.Humanarterialsmoothmusclecellscansynthesizecollagen,elastin,myosin,andglycosaminoglycan.ASMCcellsrespondtovariousfactorsbyproliferatingordifferentiating.Thesecellsareusedtostudyofhumanvasculardisorders,includingangiogenesis,stroke,vascularbiologyandalsodiabetes.HumanaorticsmoothmusclecellsareinsulinresistantatthereceptorlevelbutsensitivetoIGF1andIGF2.CurcuminpreventsthemigrationofhumanaorticsmoothmusclecellsbyinhibitingofMMP-9expression.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheASMCcellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofGAPDHinASMCcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoASMCcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:

AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Transfectionreagentsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedformaximumdeliveryefficiency.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

SelectedASMC transfectionreagentcitationreferences:

• FEBSJ.2016.283:1767–1781.TransforminggrowthfactorB1antagonizesthetranscription,expressionandvascularsignalingofguanylylcyclase…Senetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpreclinicalresearch,INDapplications,anddrugdevelopment.OurbiologyCROservices include:Xenograftservices(over60xenograftmodels),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1707)
• 1.5ml(Catalog#1708)
• 1.5mlCRISPR(Catalog#2108)
• 8.0ml(Catalog#7022)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。