Description

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TransfectionReagentfor AGSCells(GastricAdenocarcinoma,CRL1739)

• Abiodegradablepolymerbasedtrasfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• AchieverobustsiRNAuptakefordependablegenesilencing
• Effectivetransfectionunderconditionsofupto40%serum
• TransfectionkitincludesTransfectionEnhancerreagent
• Gentleenoughtobeusedforsinglecellanalysis
• DownloadinvitroAGStransfectionprotocol:[PDF]
• Download AGSCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforAGScellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast77%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsAGSTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

AGSCellLine:

Gastriccancerremainsdifficulttocureandisconsideredthethirdprimarycauseofdeathfromcancerworldwide.Becauseofthelackofearlysymptoms,itishardtodetectandisoftendiagnosedatanadvancedstage.Inhibitionofhistonedeacetylase(HDAC)wasfoundtosuppressthegrowthofgastricadenocarcinomacells,asperthe2015studypublishedintheInternationalJournalofOncology.siRNAtransfectionwasoneoftheresearchmethodsutilizedtoexploretheprogressofHDACregulationinthecancercells.TheAGSgastricadenocarcinomacelllineisextensivelyemployedinpreclinicalstudiesofstomachcancer.TheAGScelllinewasinitiallyestablishedin1979fromthestomachtissueofa54-year-oldCaucasianfemalewhosufferedfromgastricadenocarcinoma(stomachcancer).ThesecellsexhibitepithelialcellmorphologyandaretumOrigenic.Thepatientreceivednopriortherapybeforeremovalofthesecells.AGScellscouldbeusedforresearchrelatedtodifferentformsofstomachcancer.AltogenBiosystemsprovidesahighlysensitivepolymer-basedtransfectionreagentkitfortheAGSstomachcancercellline.

Data:

Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheAGScellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).

Figure2.ProteinexpressionofGAPDHinAGScells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoAGScellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1172)
• 1.5ml(Catalog#1173)
• 1.5mlCRISPR(Catalog#2105)
• 8.0ml(Catalog#1174)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。