Description

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TransfectionReagentfor 4T1Cells(BreastCancerCells,CRL-2539)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• Downloadinvitro4T1transfectionprotocol:[PDF]
• Download 4T1CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationfor4T1 cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:
Reagentexhibitsatleast67%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.

TransfectionProtocolandMSDS:
DownloadAltogenBiosystems4T1TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]

4T1CellLine:
Triple-negativebreastcancerisanuncommonsubtypeofbreastcancerthatlacksreceptorsforestrogen,progesterone,orHER2.Itisaninvasivetypeofcancerwithlimitedtreatmentoptions.The4T1triple-negativecelllinewasestablishedfromthetumorofthemammaryglandinamouse(Musmusculus).Thiscelllinewasselectedasresistantto6-thioguaninewithoutmutagentreatment.Thistypeoftumoreasily,andquicklyspreadsthroughoutthebodyofthemiceiteffects.ThistumOrigeniccelllineexhibitsepithelialcellularmorphology.Thesecellsareusefulmodelincancerresearch.The4T1celllineisespeciallyvaluableforstageIVhumanbreastcancerstudiessincetumorfromthiscelllinemimicshumanbreastcancerandeasilymetastasizesthroughmice,makingasuitablebreastcancermodel.The4T1murinebreastcancercelllineistransplantableandhastheabilitytoexhibitsimilaraggressivephenotypetothehumanbreastcancer.AltogenBiosystemsofferspre-optimizedtransfectionkitsforthe4T1cellline.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinthe4T1cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).

Figure2.ProteinexpressionofCyclophilinBin4T1cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedinto4T1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfectionproducts, elecroporationkits,andinvivodeliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1163)
• 1.5ml(Catalog#1164)
• 1.5mlCRISPR(Catalog#2208)
• 8.0ml(Catalog#7017)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。