Description

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TransfectionReagentforKeratinocyteCells(Keratinocytes)

• Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
• Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
• KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
• Hightransfectionefficacyinthe presenceofserum
• ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
• ReproducIBLetransfectionresults
• Workswellforstandardreversetransfectionandhigh-throughputapplications
• DownloadinvitroKeratinocytetransfectionprotocol:[PDF]
• Download KeratinocyteCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforKeratinocyte cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast85%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsKeratinocyteTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Keratinocyte CellLine:

Keratinocytesarethemostprevalenttypeofskincells,comprisingnearly95%oftheepidermis.Keratinocytecellsoriginateinthebasalskinlayerandgrowdenselyoutwardthroughthelayersoftheepidermis.Keratinocytecellsarecontinuouslyshedandreplacedfromtheouterlayer.Theyareconsideredthebuildingblocksofskinbecausetheyareresponsibleforkeratinproduction,thekeycomponentofhairandnails.Keratinocytecellsaretightlyinterwovenandformseamsbetweenthenervesandunderlyingtissuesoftheepidermis.Thisallowsthemtocreateaprotectivebarriertopreventinfectioussubstancescomingintothebodythroughtheskin.Also,keratinocytesplayanessentialroleinwoundhealing,demonstratingahigherexpressionofPDGFA(fibroblaststimulatingplatelet-derivedgrowthfactor)thanfibroblastsandregulatingfibroblastactivity.

Keratinocytesareusedtodevelopinvitrotechniquestoassessskinirritants.ThecellsarealsotheprincipalsourceoftheproinflammatorycytokineIL-1intheepidermis. StudieshavefoundthatBcl-2overexpressionprotectskeratinocytesfromUkrain-inducedapoptosis.Keratinocytesaresometimesdividedintosmallercelltypes,suchasbasalkeratinocytes,whicharelocatednearthebasallayer.Therearemultiplebiomedicalresearchapplicationsusingkeratinocytes.AltogenBiosystemsoffersadvancedcationiclipid-basedKeratinocytetransfectionreagentkitsthatcanefficientlyaidbiomedicalresearchrelatedtokeratinocytes.ThesekitscanbeutilizedforthedevelopmentofstableKeratinocytecelllinesandyieldhightransfectionefficiency,asdetectedbyqRT-PCR.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinthecellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).

Figure2.ProteinexpressionofCyclophilinBinKeratinocytecells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoKeratinocytecellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
• BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Transfectionreagentsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedformaximumdeliveryefficiency.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1783)
• 1.5ml(Catalog#1784)
• 1.5mlCRISPR(Catalog#2164)
• 8.0ml(Catalog#7053)

Altogen Biosystems是一家开发和制造用于生命科学研究，药物发现和开发的转染试剂盒的生物技术公司。转染试剂盒针对特定癌细胞系和原代细胞培养进行了优化，可将生物分子有效递送到靶组织中。通过先进的试剂配方和优化的转染方案实现体外（癌细胞系）和体内（动物组织靶向试剂、癌细胞系）递送货物分子，包括质粒DNA，各种类型的RNA（mRNA，siRNA，shRNA，microRNA），蛋白质和小分子研究。

苏州蚂蚁淘代理Altogen Biosystems在中国的业务。Altogen Biosystems北京代理，Altogen Biosystems华东代理，Altogen Biosystems华北代理，Altogen Biosystems全国代理，Altogen Biosystems华南代理。